Emerging Technologies Topic: Antibody Selection for complex membrane targets
23 Sep 2024
Mendocino
Bi/Multispecifics
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- Antibody discovery vs complex antigens that cannot be easily purified remains a challenge.
- Does the lack of available material limit the targets that are pursued?
- Would a consistent source of properly folded, stable, clean antigen source improve the success rates for these discovery campaigns?
- There are some options available (VLPs, Nanodiscs, DSCLs, SMALPs), however each has its limitations.
- VLPs in particular are limited by the incorporation of cellular proteins which can result in considerable background during immunization and selection
- Nanodiscs- expose scaffold and intracellular epitopes
- DSCLs- detergent solubilization can affect native confirmation
- SMALPs-pH sensitivity, precipitation, detergent use
- What are the best technologies and solutions to improve the material used for these projects?
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