Purification bottlenecks after solid phase synthesis, strategies for managing highly impure or difficult oligonucleotides and conjugates
30 Apr 2026
Quality Control
- Framing the bottleneck: Align on the most common purification failure points after the generation of solid-phase synthesis crude and how they vary by oligo length, chemistry, and conjugation.
- Current purification strategies: Compare real-world experiences with chromatographic and non-chromatographic methods, focusing on resolution, scalability, and process robustness.
- Managing difficult sequences: Discuss practical decision frameworks for handling highly impure or complex oligonucleotides, including upstream fixes, complementary purification steps, and selective cleanup approaches.
- Future needs: Identify key technology and analytics gaps that could reduce purification burden and improve end-to-end process efficiency.
