Oligonucleotide therapeutics purification relies heavily on preparative-scale chromatography. Reverse phase high performance liquid chromatography (RP-HPLC) and anion exchange chromatography (AIEX) are the most widely employed techniques. This presentation will highlight oligonucleotide purification approaches using reverse phase and anion exchange DuPont™ AmberChrom™ resins. It will cover various oligonucleotide modalities, purification approaches, and introduce a new high-loading anion exchange resin for oligonucleotide purification.