Opening Address & Keynote Presentation: Large Tumor Fragment 3D Tissue Culture Enables Multiomic Characterization of Differential T cell Responses within the Native Tumor Microenvironment Across Multiple Indications
24 Sep 2024
Immune Biomarkers
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Translational Research
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Clinical Development
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Tumor Microenvironment (TME)
![Opening Address & Keynote Presentation: Large Tumor Fragment 3D Tissue Culture Enables Multiomic Characterization of Differential T cell Responses within the Native Tumor Microenvironment Across Multiple Indications](https://cdn.asp.events/CLIENT_HubXchan_638DBA0F_0C6E_8D3C_A444829FC0C4B73E/sites/HubXChange/media/libraries/partners/Farcast-Bio.png/fit-in/1500x9999/filters:no_upscale())
Dynamic interactions within the tumor microenvironment influence patient responses to immune-modulating therapies. Current preclinical models often fail to accurately capture this complexity, leading to poor translation into human clinical trials. Using our proprietary TruTumor platform, we analyzed responses to anti-PD1 therapy in head and neck cancer patients. By integrating spatial, genomic, proteomic, and cellular multi-omic analyses, we assessed immune cell populations, T-cell activity, cytokine levels, and tumor cytotoxicity. Patients were stratified into five sub-cohorts, with extremes representing the best and worst responses. High responders exhibited robust T-cell reinvigoration and increased tumor cytotoxicity, while non-responders showed immune suppression and tumor progression. Some intermediate responders demonstrated improved outcomes with anti-CTLA4 and anti-PD1 combinations, as evidenced by better effector T-cell ratios and enhanced tumor cytotoxicity. This approach provides a valuable tool for studying drug responses in human-derived tumors, offering insights into mechanisms, pharmacodynamics, and potential biosignatures for predicting responses prior to clinical trials.