Poster Presentation: Lead ID & Optimization topic: Rapid Generation of Bispecific Antibodies for High Throughput Screening with SpyLock Technology
28 Oct 2024
Formats & Scaffolds
)
The SpyCatcher SpyTag reaction is a fast and specific way to covalently link two proteins and has proven useful for the site-specific labeling and oligomerization of antibodies Here, we have developed a method to reversibly inhibit the reactivity of SpyCatcher
by creating a mutant version that reacts with a disulfide forming small molecule, thereby abolishing reactivity. Upon reaction with a reducing agent, the small molecule is removed, and reactivity is restored. We call such a lockable SpyCatcher "SpyLock". A
dimeric fusion of SpyLock and regular SpyCatcher offers an attractive route to bispecific antibodies. This approach is particularly advantageous for screening antibody pairs recognizing most suitable epitopes or with optimally matched affinities, which can
then be further assessed in their final bispecific format. The SpyLock technology seamlessly integrates with Bio Rad’s Pioneer™ Antibody Discovery Platform but can be applied to all recombinant antibodies.
by creating a mutant version that reacts with a disulfide forming small molecule, thereby abolishing reactivity. Upon reaction with a reducing agent, the small molecule is removed, and reactivity is restored. We call such a lockable SpyCatcher "SpyLock". A
dimeric fusion of SpyLock and regular SpyCatcher offers an attractive route to bispecific antibodies. This approach is particularly advantageous for screening antibody pairs recognizing most suitable epitopes or with optimally matched affinities, which can
then be further assessed in their final bispecific format. The SpyLock technology seamlessly integrates with Bio Rad’s Pioneer™ Antibody Discovery Platform but can be applied to all recombinant antibodies.
