High-quality therapeutic antibodies aren’t the product of a single experiment, but of a series of cross-validated decisions spanning the entire drug discovery process. In this talk, Mosaic shares practical lessons from running quality-first, fit-for-purpose discovery – programs that blend in vivo and in vitro approaches to deliver candidates that work in the real world. Rather than showcasing a single case study, we’ll walk stage-by-stage through the discovery workflow, and at each stage, we’ll present a short case study: the stubborn problem we hit, what didn’t work, the tactic(s) that did, and a concrete rule of thumb you can reuse to avoid similar roadblocks in the future. Attendees will leave with actionable lessons and decision criteria they can plug directly into their next campaign, whether with Mosaic or elsewhere. Let’s build better antibodies together!