The PioneerTM Antibody Discovery Platform is Bio-Rad’s new service for biotherapeutic lead generation. The new Pioneer Antibody Library is central to the Discovery Platform. Its design is informed by over 18 years of experience in antibody phage display. It was engineered to have optimal properties for the selection of therapeutic antibody candidates, including the reduction of CDR-located posttranslational modification sites for improved developability. With 92% of the clones (VH and VL combined) encoding functional antibodies, the Pioneer Antibody Library encodes 2.25×1011 unique antibodies and is one of the largest functional Fab antibody libraries ever made. The Pioneer Platform takes advantage of SpyDisplay, a novel selection system based on SpyTag technology. Selected Fab candidates can be directly used with Bio-Rad’s modular antibody assembly platform, TrailBlazerTM, allowing fast and easy screening in a variety of formats, including various IgG-like formats. Herein, we show data on the selection of antibodies against several antigens, some of them therapeutically relevant. We demonstrate that the Pioneer Platform delivers diverse high-affinity antibody lead candidates. Using anti-TIGIT antibodies as an example, we show that, for the parameters tested, the performance of our lead candidates is comparable with several antibodies currently undergoing clinical trials.